altered axonal localization with sod1 g93a expression  (Rotem Industries)

Journal: The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry

Article Title: Expanding axonal transcriptome brings new functions for axonally-synthesized proteins in health and disease

doi: 10.1177/1073858417712668

Figure Lengend Snippet: Axonal Transcriptome Profiles

Article Snippet: The reader should bear in mind that this is a rapidly evolving area and additional diseases and/or disease mechanisms linked to axonal protein synthesis will undoubtedly be uncovered in the near future. table ft1 table-wrap mode="anchored" t5 caption a7 Disease/Disorder mRNAs Altered Mechanism References Alzheimer disease ATF4 Aβ1-42 induced transcription and anterograde transport ( Baleriola and others 2014 ) Amyotrophic lateral sclerosis (ALS) Nefl Decreased transport with TDP-43 mutation ( Alami and others 2014 ) 271 decreased + 176 increased Altered axonal localization with TDP-43 A315T expression ( Rotem and others 2017 ) 80 decreased + 95 increased Altered axonal localization with SOD1 G93A expression APC mRNA ligands Altered axonal localization with FUS/TLS expression ( Yasuda and others 2017 ) Spinal Muscular Atrophy (SMA) β-actin, GAP43, Ca v 2.2 Decreased axonal levels with SMN depletion ( Fallini and others 2016 ; Jablonka and others 2007 ) Increased and decreased mRNAs SMN depletion from motor neurons substantially alters the axonal transcriptome ( Saal and others 2014 ) Neuropathic pain CREB1 Capsaicin and IL-6 injection increase intra-axonal translation through mTOR pathways ( Melemedjian and others 2010 ; Melemedjian and others 2013 ; Melemedjian and others 2014 ) Neurotropic viral infection ?

Techniques: Microarray, Cell Differentiation, Transduction, Knockdown, RNA Binding Assay, RNA Sequencing, Expressing, Control, Binding Assay

Journal: The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry

Article Title: Expanding axonal transcriptome brings new functions for axonally-synthesized proteins in health and disease

doi: 10.1177/1073858417712668

Figure Lengend Snippet: A, Where are mRNAs stored in axons? There is clear evidence for storage of mRNAs in axons, where they are somehow sequestered away from the translational machinery and kept dormant until needed. The mechanisms underlying this are not known. How does an RNP disengage from the motor proteins used for transport into axons and avoid translation of its mRNA? Binding of additional proteins to the RNP (1) or replacement of RNP proteins with other RBPs (2) are possible mechanisms. Additionally, there must be stimulus-dependent mechanisms to recruit axonal mRNAs from their storage site (3).

Article Snippet: The reader should bear in mind that this is a rapidly evolving area and additional diseases and/or disease mechanisms linked to axonal protein synthesis will undoubtedly be uncovered in the near future. table ft1 table-wrap mode="anchored" t5 caption a7 Disease/Disorder mRNAs Altered Mechanism References Alzheimer disease ATF4 Aβ1-42 induced transcription and anterograde transport ( Baleriola and others 2014 ) Amyotrophic lateral sclerosis (ALS) Nefl Decreased transport with TDP-43 mutation ( Alami and others 2014 ) 271 decreased + 176 increased Altered axonal localization with TDP-43 A315T expression ( Rotem and others 2017 ) 80 decreased + 95 increased Altered axonal localization with SOD1 G93A expression APC mRNA ligands Altered axonal localization with FUS/TLS expression ( Yasuda and others 2017 ) Spinal Muscular Atrophy (SMA) β-actin, GAP43, Ca v 2.2 Decreased axonal levels with SMN depletion ( Fallini and others 2016 ; Jablonka and others 2007 ) Increased and decreased mRNAs SMN depletion from motor neurons substantially alters the axonal transcriptome ( Saal and others 2014 ) Neuropathic pain CREB1 Capsaicin and IL-6 injection increase intra-axonal translation through mTOR pathways ( Melemedjian and others 2010 ; Melemedjian and others 2013 ; Melemedjian and others 2014 ) Neurotropic viral infection ?

Techniques: Binding Assay